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Purpose:
To address whether chronic nutrient additions changed community structure and ecosystem productivity of a native tallgrass prairie.
Methods:
Experimental Design: In 2002, a 30x40 m area was divided into 5x5 m plots arrayed in a contiguous 6x8 plot grid. Starting in 2003, 2 nitrogen (0 and 10 g m-2) and 4 phosphorus (0, 2.5, 5, and 10 g m-2) treatments were applied to the plots in a fully factorial design (8 treatment combinations). There were 6 replicates of each treatment combination resulting a total of 48 plots. Nutrients were added by hand in an even distribution in early June. Nitrogen was added as ammonium nitrate, and phosphorus as superphosphate.
Location of Sampling Stations: Watershed 2C
Frequency of Sampling: Annually. The site is ungrazed by bison and is burned in odd years (2003, 2005, 2007, ect.)
Treatment codes are as follows: N1P0 - N0-P0, N1P1 - N0-P2.5, N1P2 - N0-P5, N1P3 - N0-P10, N2P0 - N10-P0, N2P1 - N10-P2.5, N2P2 - N10-P5, N2P3 - N10-P10.
Plant community composition: Within each plot, permanent species composition plots were designated. Species composition plots were 0.5x2 m long and were divided into 4, 0.5x0.5 m subplots. In each subplot, percent aerial cover was estimated to the nearest 1% for each species that was rooted in the plot in early June and late August. Maximum cover estimates for each species were averaged across the four quadrats for each plot, which is the data here.
Aboveground productivity: Each September, above-ground biomass was clipped to ground level within 2 20X50 cm quadrats randomly located in each plot and sorted into graminoids (grasses and sedges), non-graminoid forbs, woody plants, and previous year's dead (in years when there was no burn). Care was taken to not resample areas that were previously clipped and the permanent species composition plots were never clipped. After clipping, biomass was dried at 60oC for ca. 48 hours and then weighed. Previous year's dead biomass (unburned years) was not included in estimates of annual productivity.
Note: Pretreament data in 2002. Starting in 2003, 0 or 10 g m-2 N added and 0, 2.5, 5, or 10 g of P g m-2 added in a fully factorial design. Experiment is located in watershed 2C, species community composition data and productivity data are collected annually. Additionally, mycorrhizal root colonization data and soil nutrient data has been collected sporadically. If anyone is interested in the mycorrhizal or nutrient data they should contact M. Avolio.
Note: All plant species composition data (PPL011) missing in 2020.
For additional metadata information see: http://lter.konza.ksu.edu/sites/default/files/DC.pdf
For additional methods information see: http://lter.konza.ksu.edu/sites/default/files/MM.pdf
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